Saturday, March 9, 2019
Most Effective Hand Cleaner
Most Effective Hand Cleaner Introduction The purpose of this look into is to (a) determine if impart soap or alcoholic beverageic drink mousse is the close to impelling in killing Staphylococcus aureus (b) obtain the statistical departure of effectiveness in killing S. aureus between give way soap and alcohol colloidal gel. The null surmise is that neither hand soap nor alcohol gel is effective in killing S. aureus much than the control conference which is zip at all. The bound hypothesis is that hand soap versus the control chemical group will be more(prenominal) than effective in killing S. ureus and/or alcohol gel versus the control group will be more effective in killing S. aureus. Another alternate hypothesis is that alcohol gel is more effective in killing S. aureus than the hand soap. Materials and Methods The female genitalia of a Trypticase Soy Agar (TSA) plate is divided into three (3) pie sections along with the experimentalist initials, class day and cla ss time. A sample of S. aureus is obtained in a closed lid sample tube. The sample tube is rolled thorn and forth to suspend the organism evenly while wearing gloves.The aseptic swab is dipped into the test tube to obtain S. aureus, and then closed. The petri kayo lid is opened just long enough to gently move over the swab in a streak pattern rotating the plate at different angles. The lid is position on the petri dish and the contaminated swab is disposed in the biohazard bag. A blank disk is placed in the centre of section one (1) with sterile tweezers. A stake disk is dipped into hand soap and placed in the center of section two (2).A third disk is dipped into alcohol gel and placed in the center of section three (3). for each one disk is softly tapped into media to secure. The petri dish is placed bottom side up in an incubator set at 37 degrees Celsius for three (3) geezerhood and then placed into refrigerator. Two (2) weeks later the diameter of the regulate of cha nge around the disk from each section is measured in millimeters. The info is collected from each class and is compiled to calculate the mean, standard deviation, and SEM.The compiled data is canvas by creating a bar graph to compare the zone of clarification for each group. Results DATA CLASS STASTICS Treatment Groups Clear Zone (mm) suppose (mm) Standard Deviation SEM Error Bars Mean + (1. 96) SEM Mean (1. 96) SEM 1. unfilled Disk 0 0 0 0 0 0 2. Hand Soap 15 11. 21 3. 65 0. 74 1. 46 1. 46 3. Alcohol Gel 15 13. 21 3. 93 0. 80 1. 57 1. 57 Discussion and Conclusion There was a remarkable difference between the control group and the treatment groups.The control group had no effect on killing S. aureus, but the treatment groups did select an effect on killing S. aureus. The hand soap mean zone of clearing was 11. 21mm and the alcohol mean zone of clearing was 13. 21mm. The null hypothesis that neither hand soap nor alcohol gel is effective in killing S. aureus more than th e control group is rejected. The alternate hypothesis that hand soap versus the control group is more effective killing S. aureus is accepted.The alternate hypothesis that alcohol gel versus the control group is more effective in killing S. aureus is accepted. The alternate hypothesis that alcohol gel is more effective than the hand soap in killing S. aureus is rejected. The data suggests hand soap is not as effective as alcohol gel however, statistically, the data does not support that alcohol gel is a more effective treatment because the error bars overlap. In conclusion, cleaning pass on with either hand soap or alcohol gel is break dance at killing S. aureus than using nothing at all.
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